System Validation

When it comes to choosing a PRP and Stem Cell isolation system, the most important question to ask is: Is the system validated for use in Canine patients? There are numerous isolation systems available, but very few are specifically validated for use in dogs.

The Companion Regenerative Therapies (CRT) system has been specifically validated for use in canine patients. In a multicenter study*, the CRT Pure PRP system was the only system that increased platelet concentration while significantly reducing the red blood cell and neutrophil concentrations.

Companion Regenerative Therapies validation charts

Why is it Important to Reduce Neutrophils and Red Blood Cells (RBCs)?


  • Multiple studies show that neutrophils increase concentrations of unwanted inflammatory mediators (IL-1β, TNF-α, IL-6, IL-8)
  • Increased concentrations of neutrophils in PRP is positively correlated with an increased MMP-9 concentration, which leads to degradation of collagen and other extracellular matrix molecules

Red Blood Cells:

  • RBC’s damage cartilage and synovium directly via iron-catalyzed formation of Reactive Oxygen Species (ROS)
  • RBC’s increase concentration of unwanted inflammatory mediators (IL-1 and TGF-α)
  • PRP with high concentration of RBC’s caused significantly more synoviocyte death when compared to PRP preparations with lower RBC count

For more information about Companion Regenerative Therapy, please contact Companion and we will provide you with additional information about our industry-leading education and canine-validated technology.

Braun HJ, et al., Am J Sports Med. 2014
Sundman EA, et al. Am J Sports Med. 2013

* Carr, B, Canapp, S. et al. “Canine Platelet Rich Plasma Systems: A Multicenter, Prospective Analysis.” (2015).

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